|
KMID : 0861020210360050059
|
|
Korea Journal of Herbology
2021 Volume.36 No. 5 p.59 ~ p.67
|
|
|
Investigation of the effect of Hibiscus sabdariffa L. extracts on tight-junction related genes in human keratinocyte HaCaT cells
|
|
Jung Hae-Soo
Kim Eun-Mi
Han Hyo-Sang
Kim Kee-K.
|
|
|
Abstract
|
|
|
Objectives : Hibiscus (Hibiscus sabdariffa L.) is rich in antioxidants such as flavonoids and anthocyanins and is known to have anti-inflammatory activity and anti-aging function of the skin, but there is no study on its effect on the skin barrier. This study aim to investigate the positive effect on the skin barrier by confirming the effect of water extracts of Hibiscus sabdariffa L. (WEHS) on the tight junction-related gene expression.
Methods : The antioxidant efficacy of WEHS was investigated through ABTS and DPPH assays, and 3-(4,5- dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium was performed to examine the effect on cell viability. quantitative Reverse transcription polymerase chain reaction and immunoblot analysis were performed to confirm the effect of WEHS on the expression of tight junction-related genes, and immunofluorescence microscopy was used to confirm the movement of Claudin 1 protein into tight junctions.
Results : WEHS showed strong antioxidant activity and induced an increase in both mRNA and protein expression levels of Claudin 1 among tight junction-related genes. The strong localization of Claudine 1 protein increased by WEHS to the tight junction was confirmed by immunofluorescence microscopy.
Conclusions : Hibiscus was confirmed through this study to show antioxidant activity and the function of promoting the expression of the tight junction Claudin 1 gene, suggesting that Hibiscus can be used as a material for the prevention and treatment of skin moisturizing and atopy, which have an important influence on tight junction.
|
|
|
KEYWORD
|
|
|
Hibiscus sabdariffa L, Tight junction, Claudin 1, HaCaT cell, Antioxidant
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|